Isolation and Partial Characterization of Virus Inhibitors From Plant Species Taxonomically Related to Phytolacca

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Abstract
A procedure was developed for obtaining a proteinaceous virus inhibitor from P. americana in an electrophoretically pure state. The final steps of the procedure involved adsorption to and elution from the cation exchange resin CM [carboxymethyl]-Sephadex, followed by passage through DEAE-Sephadex under nonadsorbing conditions. When the same procedure was applied to 14 other plant species reported to contain virus inhibitors, including several species of the order Centrospermae which is believed to be phylogenetically derived from the Phytolaccaceae, similar biologically active proteins were obtained in every case. In tests to evaluate further similarities only 2 of 8 spp. of the Centrospermae yielded substantial amounts of virus inhibitors that had the same MW and were serologically related to the Phytolacca protein.