Vitamin B12and Marine Ecology III. An experiment with a chemostat

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Abstract
A 250 ml. chemostat has been used to measure the parameters of vitamin B requirement in the chrysomonadMonochrysis lutheri.The yield constant wasca.0·25 × 10 cells/μμg., i.e. one-third of the figure previously measured in batch cultures.The saturation constant lay between 2 and 6 μμ/ml., i.e. at least twenty times the figure indicated by previous batch culture measurements.Internal inconsistencies in the chemostat's operation indicate the presence of factors not accounted for by theory.It is argued that only factors analogous to the release of protein-bound vitamin into the medium could in principle account for all the discrepancies.