Monolayer Tissue Cultures I. Preparation and Standardization of Suspensions of Trypsin-Dispersed Monkey Kidney Cells.

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Abstract
An adaptation of the method of Dulbecco for prepn. of trypsin-dispersed monkey kidney cell suspension has been descr. The modifications include (a) use of mechanical rather than manual agitation for dispersing the cells, (b) use of a different nutrient medium, and (c) quantitative adjustment of the dispersed cell suspension on the basis of total cell count or optical density. Procedures employed yield quantitatively standardized cell suspensions which can be used in the prepn. of large nos. of replicate monolayer cultures.

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