Elimination of double strand nuclease activity from S1 nuclease prepared from crude amylase
Open Access
- 1 May 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (5) , 1419-1424
- https://doi.org/10.1093/nar/3.5.1419
Abstract
Single strand-specific S1 nuclease prepared as previously described from crude α amylase by DEAE-cellulose chromatography also contains nuclease which degrades double strand nucleic acid. The double strand activity can be removed by repeating the DEAE-cellulose chromatography procedure at least two additional times. S1 nuclease prepared by this procedure does not degrade double strand sheared DNA as measured by Sephadex chromatography. Under the same conditions single strand DNA is completely degraded. Thus, S1 nuclease prepared by this procedure is suitable for use in removing single strand regions in DNA/DNA duplexes and DNA/RNA hybrids.Keywords
This publication has 9 references indexed in Scilit:
- Purification of SI nuclease from takadiastase by affinity chromatography on single-stranded DNA-acrylamide columnsNucleic Acids Research, 1975
- Purification and Further Properties of Single‐Strand‐Specific Nuclease from Aspergillus oryzaeEuropean Journal of Biochemistry, 1973
- Determination of protein: A modification of the lowry method that gives a linear photometric responseAnalytical Biochemistry, 1972
- A crude nuclease preparation suitable for use in DNA reassociation experimentsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Mung bean nuclease I. II. Resistance of double stranded deoxyribonucleic acid and susceptibility of regions rich in adenosine and thymidine to enzymatic hydrolysis.1970
- Specific inhibition by ATP and other properties of an endonuclease of Neurospora crassaCanadian Journal of Biochemistry, 1968
- A nuclease specific for heat-denatured DNA isolated from a product of Aspergillus oryzaeBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE .I. PURIFICATION AND PROPERTIES OF ENZYME1965
- Characterization of a deoxyribonuclease of Mustelus canis liverBiochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1965