A COMPARISON OF METHODS FOR EXTRACTING PITUITARY ANTIDIURETIC SUBSTANCE FROM URINE

Publisher Website
Google Scholar
Abstract
TRINARY excretion of an antidiuretic substance, presumably of posterior lobe origin, has been studied by numerous workers. Such a substance has been reported to be present in a variety of conditions such as dehydration (1), toxemia of pregnancy (2, 3), and hypertension (4). The most frequently used method of extracting the antidiuretic substance has been dialysis either with a cellophane membrane or collodion (1, 5, 6, 7). Dialysis methods of extraction have been criticized on the grounds of inefficiency (8, 9), and variability (10). In 1939, Noble, Rindernecht and Williams (11) proposed a method of extracting posterior lobe antidiuretic hormone ADH from urine, where the activity is adsorbed on zinc ferrocyanide and eluted with a solution of ammonia in ethyl alcohol. This method of extraction has been used in this laboratory for several years and has been found to recover consistently from 70% to 90% of the antidiuretic activity of ADH in urine.