A simple strategy to amplify specifically the HLA‐DQβ gene region with genomic DNA as template
- 15 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 236 (1) , 23-26
- https://doi.org/10.1016/0014-5793(88)80278-3
Abstract
The nature of codon 57 in the HLA‐DQβ gene was recently reported as a potential marker of genetic susceptibility to insulin‐dependent diabetes mellitus. When exploring the relevance of this marker by using genomic DNA amplification, we encountered difficulties resulting from the coamplification of the homologous DXβ region. A simple strategy is proposed to amplify the DQβ region exclusively. It involves the preliminary digestion of genomic DNA with a restriction enzyme which cleaves DXβ specifically, leaving intact the DQβ sequence. The amplified material is suitable for dot blot analysis and restriction enzyme digestion. This strategy is of general interest when homologous sequences impair the specificity of enzymatic DNA amplification.Keywords
This publication has 14 references indexed in Scilit:
- A simple salting out procedure for extracting DNA from human nucleated cellsNucleic Acids Research, 1988
- Direct sequencing of enzymatically amplified human genomic DNA.Proceedings of the National Academy of Sciences, 1988
- Characterization of β-thalassaemia mutations using direct genomic sequencing of amplified single copy DNANature, 1987
- HLA-DQβ gene contributes to susceptibility and resistance to insulin-dependent diabetes mellitusNature, 1987
- A rapid method for the purification of DNA from bloodNucleic Acids Research, 1987
- Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probesNature, 1986
- Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic SequencesScience, 1986
- CYCLOSPORIN INCREASES THE RATE AND LENGTH OF REMISSIONS IN INSULIN-DEPENDENT DIABETES OF RECENT ONSETThe Lancet, 1986
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- Isolation of High‐Molecular‐Weight DNA from Mammalian CellsEuropean Journal of Biochemistry, 1973