Measurement of immunoglobulin A, G, and M class rotavirus antibodies in serum and mucosal secretions

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Abstract
A solid-phase, enzyme-linked immunospecific assay for measurement of different immunoglobulin classes of human rotavirus antibodies is described. The antigen, which was adsorbed directly to polyvinyl microtiter plates, consisted of a clarified cell culture stock of the simian rotavirus SA 11. The assay was sensitive and reproducible and could readily be calibrated to determine concentrations of each class of antibody. The assay was applied to measurements of rotavirus antibodies in serum, colostrum, milk and fecal samples. It particularly facilitates investigations of the role of IgA antibodies in immunity to rotavirus infections.