ISOLATION AND IDENTIFICATION OF 17 -OESTRADIOL IN THE BILE OF A PREGNANT COW
- 1 April 1965
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 9 (2) , 137-147
- https://doi.org/10.1530/jrf.0.0090137
Abstract
Free 17[alpha]-estradiol has been isolated and identified in a pregnant cow''s bile. The identification was based on several specific color reactions, the behavior of different derivatives of the compound in alumina and thin-layer chromatography and finally on a great number of gas chromatographic investigations. A sensitive and highly specific color test for 17[alpha]-estradiol and its derivatives in thin-layer chromatograms, making use of phosphomoly-bdic acid, has been presented.Keywords
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