Steroids and other lipids of pregnant goat's urine

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Abstract
Pregnant goat''s urine was hydrolyzed with acid, and the lipid material obtained was submitted to the fractionation procedures customary in the study of urinary steroids. Estrone (0.3 mg/l) and estradiol-17 (0.1 mg/l) were isolated from the phenolic fraction. Estradiol-17 could not be detected. The heterocyclic phenol equol (isoflavan-7:3 -diol) was also isolated (10-15 mg/1). 5-Pregnane-3:20 -diol (2 mg/1), the common "pregnanediol" of human pregnancy urine, was isolated from the neutral non-ketonic fraction. There was no evidence for the presence of any isomeric pregnanediols. The neutral non-volatile ketonic fraction was small (1 mg/l). EpiAndrosterone (3-hydroxy-5-androstan-17-one) was isolated from it in impure form. Three other -17-Oxo steroids were partially characterized. Much other volatile material giving a purple colour in the Zimmermann dinitrobenzene reaction was present. This "apparent 17-ketosteroid" material probably includes ionine derivatives Holtz, 1954). Species differences in urinary steroids are discussed.