Accumulation of protoporphyrin IX in light‐sensitive mutants of Escherichia coli
- 5 October 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 310 (3) , 246-248
- https://doi.org/10.1016/0014-5793(92)81341-i
Abstract
The accumulation of protoporphyrin IX (Proto IX) in light‐sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wild‐type strain CA274 or in visA mutants that had been rendered light‐resistant by introduction of the wild‐type visA + gene. Accumulation of Proto IX was also not observed in cells with a mutation in the visB gene. These results confirm the hypothesis that the sensitivity of the visA mutants to light is caused by the abnormal accumulation of Proto IX, a substrate of ferrochelatase, as the result of a genetic defect in the gene for ferrochelatase.Keywords
This publication has 7 references indexed in Scilit:
- Photosensitivity of a protoporphyrin-accumulating, light-sensitive mutant (visA) of Escherichia coli K-12.Proceedings of the National Academy of Sciences, 1991
- Isolation and characterization of visible light-sensitive mutants of Escherichia coli K12Journal of Molecular Biology, 1991
- Diphenyl ether herbicide-decreased heme contents stimulate 5-aminolevulinic acid synthesisPesticide Biochemistry and Physiology, 1990
- [1] Production of single-stranded plasmid DNAPublished by Elsevier ,1987
- Viable Molecular Hybrids of Bacteriophage Lambda and Eukaryotic DNAProceedings of the National Academy of Sciences, 1974
- Duplicate genes for tyrosine transfer RNA in Escherichia coliJournal of Molecular Biology, 1970
- Role of Lysosomes and of Cell Membranes in PhotosensitizationNature, 1966