An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA.
- 1 February 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (2) , 799-803
- https://doi.org/10.1073/pnas.77.2.799
Abstract
Protein n'', a prepriming DNA replication enzyme of E. coli, is a .vphi.X174 DNA-dependent ATPase. Restriction endonuclease fragmentation and exonuclease VII digestion of .vphi.X174 DNA led to the identification of a 55-nucleotide fragment that carries the protein n'' recognition sequence. Molecular hybridization and sequence analysis located this sequence within the untranslated region between genes F and G, a map location analogous to that of the unique complementary strand origin of phage G4 DNA. Within the 55-nucleotide fragment is a sequence of 44 nucleotides that forms a stable hairpin structure. This duplex may be the signal for protein n'' to initiate the prepriming events that lead to the start of .vphi.X174 complementary DNA strand replication.This publication has 26 references indexed in Scilit:
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