A new method for sequencing DNA.
Open Access
- 1 February 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (2) , 560-564
- https://doi.org/10.1073/pnas.74.2.560
Abstract
DNA can be sequenced by a chemical procedure that breaks a terminally labeled DNA molecule partially at each repetition of a base. The lengths of the labeled fragments then identify the positions of that base. We describe reactions that cleave DNA preferentially at guanines, at adenines, at cytosines and thymines equally, and at cytosines alone. When the products of these four reactions are resolved by size, by electrophoresis on a polyacrylamide gel, the DNA sequence can be read from the pattern of radioactive bands. The technique will permit sequencing of at least 100 bases from the point of labeling.This publication has 12 references indexed in Scilit:
- Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferaseNucleic Acids Research, 1976
- A specific endonuclease from Arthrobacter luteusJournal of Molecular Biology, 1976
- Polynucleotide. CXXIV. Reversal of bacteriophage T4 induced polynucleotide kinase actionBiochemistry, 1973
- Gel electrophoretic separation of the complementary strands of bacteriophage DNAVirology, 1972
- The degradation of DNA by hydrazine: A critical study of the suitability of the reaction for the quantitative determination of purine nucleotide sequencesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Hydrazinolysis of some purines and pyrimidines and their related nucleosides and nucleotidesJournal of the Chemical Society C: Organic, 1967
- Studies on the nucleotide arrangement in deoxyribonucleic acids IX. Selective degradation of pyrimidine deoxyribonucleotidesBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1964
- Methylation of deoxyribonucleic acid by diazomethaneBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1964
- A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activityBiochemical Journal, 1964
- FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDESBiochemical Journal, 1963