A Simple Procedure for the Study of Ionic Regulation in Small Animals

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Abstract
A simple procedure is described for the estimation of sodium, potassium, calcium and magnesium in quantities of about 1 µg. The ions are precipitated by specific chemical reagents, the precipitates separated by centrifugation and converted quantitatively into chlorides. The chloride is titrated by the Volhard method or by an electrometric method using a simple automatic micro-burette. Measurements made on simple salt solutions and on blood samples gave standard deviations of 1-2% except in the sodium method where the error was greater (2-4%).