Single DNA molecule grafting and manipulation using a combined atomic force microscope and an optical tweezer
- 22 December 1997
- journal article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 71 (25) , 3727-3729
- https://doi.org/10.1063/1.120495
Abstract
In this letter, we report on spatially selecting and grafting a DNA-tethered bead to an atomic force microscope (AFM) cantilever, using an optical tweezer. To quantify this technique, we measure force versus extension of a single DNA molecule using AFM. For such studies, we have developed a micromanipulation approach by combining an AFM, an optical tweezer, and visualization setup. The ability to select a single DNA polymer and specifically graft it to a localized position on a substrate opens up new possibilities in biosensors and bioelectronic devices.This publication has 9 references indexed in Scilit:
- Stretching DNA with optical tweezersBiophysical Journal, 1997
- Quantitative measurements of force and displacement using an optical trapBiophysical Journal, 1996
- Overstretching B-DNA: The Elastic Response of Individual Double-Stranded and Single-Stranded DNA MoleculesScience, 1996
- DNA: An Extensible MoleculeScience, 1996
- Stretching DNAMacromolecules, 1995
- An atomic-resolution atomic-force microscope implemented using an optical leverJournal of Applied Physics, 1989
- Novel optical approach to atomic force microscopyApplied Physics Letters, 1988
- Observation of a single-beam gradient force optical trap for dielectric particlesOptics Letters, 1986
- Atomic Force MicroscopePhysical Review Letters, 1986