Differential expression of laminin chains in hepatic lipocytes
- 23 September 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 290 (1-2) , 9-12
- https://doi.org/10.1016/0014-5793(91)81213-r
Abstract
The lipocyte is an important source of laminin in the normal liver. We have investigated the expression of the 3 chains of laminin in isolated rat lipocytes. Both B1 and B2 chains, but not A, were found in medium from 5-day-old lipocyte primary cultures by immunoblotting and immunoprecipitation of 35S-labeled proteins after reducing SDS-polyacrylamide gel electrophoresis. An additional polypeptide of Mr=380 000 was identified by immunoprecipitation. Under non-reducing conditions only one Mr=900 000 band was revealed. High levels of B1 and B2 mRNAs were also demonstrated in 5-day-old cultured lipocytes while at the time of seeding, only B2 chain mRNAs were clearly detectable. A chain mRNA was constantly absent. These results suggest that lipocytes produce a variant form of laminin in primary culture and that the Mr=380 000 polypeptide could be unrelated to the A chain of lamininKeywords
This publication has 22 references indexed in Scilit:
- Merosin, a tissue-specific basement membrane protein, is a laminin-like protein.Proceedings of the National Academy of Sciences, 1990
- Mouse heart lamininPublished by Elsevier ,1989
- A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junctionNature, 1989
- Differential expression of the laminin A and B chains in chimeric kidneysCell Differentiation, 1988
- Hepatocytes may produce laminin in fibrotic liver and in primary cultureHepatology, 1988
- Laminin and Other Basement Membrane ComponentsAnnual Review of Cell Biology, 1987
- Genes for basement membrane proteins are coordinately expressed in differentiating F9 cells but not in normal adult murine tissuesDevelopmental Biology, 1987
- Laminin — a multifunctional protein of basement membranesTrends in Biochemical Sciences, 1983
- Isolation of laminin from human placental basement membranes: Amnion, chorion and chorionic microvesselsBiochemical and Biophysical Research Communications, 1983
- Subunits of laminin are differentially synthesized in mouse eggs and early embryosDevelopmental Biology, 1983