Method for sequential staining of GTL‐banded metaphases with fluorescent‐labeled chromosome‐specific paint probes

Abstract
We describe a method for use of fluorescent‐labeled whole chromosome‐specific paint probes on GTL‐banded metaphases to utilize the combined potential of these techniques for defining chromosome abnormalities. The efficacy of this method was tested on 6 cases involving different chromosome abnormalities and various tissues, including blood, amniotic fluid, skin fibroblasts, and bone marrow.