Partial purification of a factor from human synovium that possesses interleukin 1, chondrocyte stimulating and catabolin‐like activities
- 7 January 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 179 (2) , 247-251
- https://doi.org/10.1016/0014-5793(85)80528-7
Abstract
Human synovial explants in culture release material that stimulates the production of prostaglandin E2 (PGE2) and several extracellular enzymes by human chondrocytes. Fractionation of conditioned medium by gel filtration revealed a protein of approx. 15 kDa, which in addition to stimulating production of PGE2 and plasminogen activator by human articular chondrocytes, possessed interleukin 1 activity and induced cartilage degradation. Further purification using iso‐electric focussing again showed co‐elution of these activities with a major pI of 6.9 and a minor pI of 5.1–5.3. This study indicated that human synovium releases a facor that is closely related to or identical with interleukin 1 and suggests that this protein may participate in cellular interactions that occur within the rheumatoid joint.Keywords
This publication has 19 references indexed in Scilit:
- Stimulation by human interleukin 1 of cartilage breakdown and production of collagenase and proteoglycanase by human chondrocytes but not by human osteoblasts in vitroBiochimica et Biophysica Acta (BBA) - General Subjects, 1984
- Interleukin-1Clinical Infectious Diseases, 1984
- An interleukin 1 like factor stimulates bone resorption in vitroNature, 1983
- Effects of retinol and dexamethasone on cytokine-mediated control of metalloproteinases and their inhibitors by human articular chondrocytes and synovial cells in cultureBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1983
- Human mononuclear cell factors mediate cartilage matrix degradation through chondrocyte activation.Journal of Clinical Investigation, 1981
- Stimulation of rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin 1).Proceedings of the National Academy of Sciences, 1981
- Identification of catabolin, a protein from synovium which induces degradation of cartilage in organ cultureBiochemical and Biophysical Research Communications, 1980
- Human synovium releases a factor which stimulates chondrocyte production of PGE and plasminogen activatorNature, 1980
- The Effect of Synovial Tissue on the Breakdown of Articular Cartilage in Organ CultureArthritis & Rheumatism, 1977
- Collagenase Production by Rheumatoid Synovial Cells: Stimulation by a Human Lymphocyte FactorScience, 1977