Transformation and mobilization of cloning vectors in Acinetobacter spp
Open Access
- 31 December 1985
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 165 (1) , 301-303
- https://doi.org/10.1128/jb.165.1.301-303.1986
Abstract
R300B-, RSF1010-, and RK2-derived plasmids were introduced into Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413 by transformation and conjugal mobilization. The transformation frequencies of BD413 were 4.2 X 10(6) to 6.3 X 10(6) transformants per micrograms of DNA per 10(9) recipient cells. Conjugal mobilization frequencies were 1.1 X 10(-1) to 8.5 X 10(-1) per recipient. An improved method for the transformation of A. calcoaceticus BD413 is reported.This publication has 20 references indexed in Scilit:
- Host:Vector Systems for Gene Cloning in PseudomonasPublished by Springer Nature ,1982
- Vectors for Gene Cloning in Pseudomonas and Their ApplicationsPublished by Springer Nature ,1982
- Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in PseudomonasGene, 1981
- Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.Proceedings of the National Academy of Sciences, 1980
- Improved conversion of methanol to single-cell protein by Methylophilus methylotrophusNature, 1980
- Conditions for quantitative transformation inAcinetobacter calcoaceticusCurrent Microbiology, 1979
- Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in transProceedings of the National Academy of Sciences, 1979
- RP4-mediated Conjugation in Acinetobacter calcoaceticusJournal of General Microbiology, 1976
- SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORMProceedings of the National Academy of Sciences, 1969
- Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in escherichia coli K12Biochemical and Biophysical Research Communications, 1965