A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454
Open Access
- 5 February 2010
- journal article
- method
- Published by Springer Nature in Genome Biology
- Vol. 11 (2) , R15
- https://doi.org/10.1186/gb-2010-11-2-r15
Abstract
We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method.Keywords
This publication has 23 references indexed in Scilit:
- The NIH Human Microbiome ProjectGenome Research, 2009
- Amplification-free Illumina sequencing-library preparation facilitates improved mapping and assembly of (G+C)-biased genomesNature Methods, 2009
- Ab initio construction of a eukaryotic transcriptome by massively parallel mRNA sequencingProceedings of the National Academy of Sciences, 2009
- A large genome center's improvements to the Illumina sequencing systemNature Methods, 2008
- Comprehensive genomic characterization defines human glioblastoma genes and core pathwaysNature, 2008
- Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplexNature Methods, 2008
- From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencingNucleic Acids Research, 2007
- A pyrosequencing-tailored nucleotide barcode design unveils opportunities for large-scale sample multiplexingNucleic Acids Research, 2007
- Ultrasound–biophysics mechanismsProgress in Biophysics and Molecular Biology, 2007
- Functional profiling of the Saccharomyces cerevisiae genomeNature, 2002