PRESERVATION OF ISCHEMICALLY INJURED CANINE KIDNEYS BY RETROGRADE OXYGEN PERSUFFLATION

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Abstract
Canine kidneys were briefly perfused with Ross and Marshall''s hypertonic citrate solution and stored at 0.degree. C. The effect, during such storage, of insufflating various gases via the renal vein and allowing the gas to escape through needle perforations of the renal surface was studied. The finding of Ross and Escott was confirmed; kidneys that have suffered 30 min of warm ischemia prior to preservation, will, if O2 is so persufflated during 24 h storage, provide life-supporting function when subsequently autografted. The preservation period was extended to 48 h after 30 min warm ischemia; 24-h preservation after 60 min of warm ischemia was achieved. Air was less effective than pure O2; inert gases were completely ineffective. Uniformly high O2 tensions were measured throughout the kidneys during storage, but resynthesis of ATP and ADP was not demonstrated. The mechanism responsible for the effectiveness of retrograde O2 persufflation remains obscure.