A NEW SIMPLE METHOD FOR OPTIMAL STORAGE OF ISCHEMICALLY DAMAGED KIDNEYS

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Abstract
SUMMARY A new method was developed for sufficient normobaric oxygenation of kidneys during storage in solutions of intracellular ionic composition. Sufficiency of oxygenation by “retrograde oxygen persufflation” was controlled by estimation of cellular metabolic state. In dogs functional recovery of the kidney was tested following autotransplantation after 24 hr of hypothermic persufflation storage, with or without a preceding period of 30 min of normothermic ischemia, and compared with simple storage in Collins' solution or continuous perfusion with albumin solution. Kidney function immediately after transplantation and 24 hr later was determined by inulin and p-aminohippurate clearances for transplanted and contralateral normal kidneys simultaneously. Immediate functional recovery values after persufflation storage amounted to 56 and 46% of the normal contralateral kidney in p-aminohippurate and inulin clearances, respectively, and were similar to the results of continuous perfusion. Furthermore, even after 30 min of normothermic ischemia followed by 24 hr of persufflation storage, the clearance values reached 49 and 32% of the normal values for p-aminohippurate and inulin, respectively, immediately after transplantation. Thus, the method of persufflation storage comines the advantages of storage and continuous perfusion it is simple and cheap like the storage methods but is most effective even in ischemically damaged organs as continuous perfusion.