Synthesis of Levan by Levansucrase
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 85 (1) , 287-293
- https://doi.org/10.1093/oxfordjournals.jbchem.a132322
Abstract
The addition of levan as a so-called “acceptor” accelerated the rate of polymerization of levan catalyzed by levansucrase [EC 2·4·1·10]. However, this effect was seen only under conditions of low ionic strength. Under conditions of high ionic strength, only levan with an average degree of polymerization (DP) of 120 was synthesized with a reasonable yield. Incorporation of [14C]fructose residues into levan of high molecular weight (DP 1,200) added as an “acceptor” at high ionic strength was slight. It is suggested that the DP of levan synthesized is generally regulated by ionic strength, and that enzymic synthesis of levan occurs in the absence of an “acceptor.”This publication has 3 references indexed in Scilit:
- Levansucrase ofBacillus subtilisAgricultural and Biological Chemistry, 1978
- The mechanism of dextransucrase actionArchives of Biochemistry and Biophysics, 1977
- Colorimetric estimation of ketopentoses and ketohexosesBiochemical Journal, 1956