Colorimetric estimation of ketopentoses and ketohexoses

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Abstract
A modification of the resorcinol reaction is described which is suitable for the estimation of ketopentoses and ketohexoses in binary mixtures provided that there is less than a twofold excess of one ketose over the other. The characteristics of the colors given with the new reagent by fructose, sorbose, tagatose, xylulose, perseulose and man-noheptulose are described. The interference due to aldoses is small. Ketoheptoses interfere appreciably with the estimation of ketohexoses and ketopentoses. Satisfactory recoveries of ketoses added to blood have been obtained by this method.