Oxytocin induced cAMP‐dependent protein kinase activation and urokinase‐type plasminogen activator production in LLC‐PK1 renal epithelial cells is mediated by the vasopressin V2‐receptor

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Abstract
Using a variety of peptide analogues of oxytocin (OT) and Arg8‐vasopressin (AVP), OT‐mediated induction of urokinase‐type plasminogen activator (uPA) was examined in LLC‐PK1 renal epithelial cells, which possess distinct high‐affinity receptors of both the OT‐ and vasopressin renal (V2‐) types. OT or OT‐receptor specific agonists induced concentration‐dependent cAMP synthesis, activation of the cAMP‐dependent protein kinase (cAMP‐PK) and uPA production consistent with their respective binding affinities for the V2‐ and not the OT‐receptor. OT‐mediated uPA induction could be inhibited in a concentration‐dependent fashion by coincubation with a V2/V1‐receptor specific antagonist, but not by an OT‐receptor specific antagonist. Results implied that stimulation of cAMP‐ and uPA responses in LLC‐PK1 cells by OT was V2‐receptor‐mediated.