TAXOL IS CONVERTED TO 7-EPITAXOL, A BIOLOGICALLY-ACTIVE ISOMER, IN CELL-CULTURE MEDIUM
- 1 August 1987
- journal article
- research article
- Vol. 242 (2) , 692-698
Abstract
The hydrolysis products of taxol have been isolated by high-performance liquid chromatography and identified by nuclear magnetic resonance and mass spectroscopy. In contrast to taxol, the major hydrolysis product, baccatin III, has little cytotoxic activity and does not promote in vitro microtubule assembly. In cell culture medium, the concentration of taxol decreases with time and 7-epitaxol, which exhibits properties comparable to those of taxol both on cells and on in vitro microtube polymerization, is formed. Baccatin III is found in small quantities in the cell medium, although it is barely detectable within the cells. It is concluded that 7-epitaxol is the major derivative of taxol found in cells and that its presence does not alter, in a major way, the overall biological activity of taxol.This publication has 12 references indexed in Scilit:
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