The use of radioactive isotopes in immunological investigations. 7. Labelled proteins and protein antigens containing both iodine and mustard gas sulphone groups

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Abstract
Methods are described for introducing into proteins both l:4-thiazan l:l-dioxide groups (by treatment with di-2-chloroethyl sulfone) and 3:5-diiodotyrosine groups. The number of the 2 groups introduced into a protein is partly dependent on the order in which the iodination and treatment with sulfone are carried out. Results are discussed with reference to the positions in the protein molecule taken up by the entering groups, and the efficacy of the method for the preparation of double-labeled protein antigens. A method of "trace-labelling" proteins with both I131 and S35 is described.