The separation and determination of microquantities of lower aliphatic acids, including fluoroacetic acid

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Abstract
Fluoroacetate can be separated from formate, acetate and other straight-chain fatty acids by paper chromatography of the hydrox-amates in ammoniacal ethanol-pyridine-water. The specific effect of ammonia is shared by mono- and dimethylamines, but is absent from trimethylamine. This may indicate the formation of an intramolecular hydrogen bond between an NH-group and fluorine. Hydroxamic acids can be determined quantitatively by direct iodine oxidation to nitrite, which is used to form a red azo dye. The method has a lower limit of 0.5 [mu]g/ml of final solution. The new procedure involves 2-dimensional paper chromatography of the hydroxamic acids, extraction, and formation of the dye.