Abstract
Lactobacilli isolated from the bovine rumen and avian Lactobacillus bifidus require pantethine and enzyme-hydrolyzed casein for optimum growth in synthetic media. The pantethine requirement is replaced by coenzyme A but not by excess pantothenic acid. Starch and the disaccharides, lactose and maltose, are utilized more readily than glucose as energy sources indicating close relationship between these types of lactic acid bacteria. The common lactobacilli use monosaccharides more readily than the higher sugars.