Long-term culture of pancreatic islet cells with special reference to the β-cell function

Publisher Website
Google Scholar
Abstract
Islet cells of adult rat pancreas, dissociated with EDTA-Dispase, were cultivated in Microtest wells for over 2 months. In our cultures, islet cells were free-floating and cohered with each other to reorganize histotypic aggregates resembling the nondissociated islets. Morphologically, excellent preservation of islet cells in the aggregates was confirmed during the culture using both light and electron microscopy. The function of islet β-cells, as demonstrated by the synthesis and release of insulin, also was retained throughout the culture period. Islet β-cells cultured for long periods exhibited better response to the shortterm stimulation of theophylline than to a high concentration of glucose, as observed in the islets of fetal or newborn rat pancreas.