Potassium and Reducing Substances in Proximal Tubule Fluid of the Rat Kidney.

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Abstract
The technique of Wearn and Richards as modified for the mammalian kidney by Walker and Oliver was used for the collection of fluid and identification of the puncture site in the kidneys of rats. The Bott method of analysis was used for K and a modification of the Walker and Reisinger method for reducing substances in tubule fluid and plasma. This modification consisted of photo-electric reading of the light absorption directly in capillary tubes. In 8 of 9 experiments K concentrations in fluid collected from the middle third of the proximal tubule were lower than in plasma, indicating that K appears to be reabsorbed actively from proximal tubule fluid. The site of the process is roughly identical with that of active glucose reabsorption.

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