Functional expression of an alpha anti‐insect scorpion neurotoxin in insect cells and lepidopterous larvae
- 4 December 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 376 (3) , 181-184
- https://doi.org/10.1016/0014-5793(95)01263-4
Abstract
The Leiurus quinquestriatus hebraeus alpha anti‐insect toxin (LqhαIT) cDNA was engineered into the Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. Insect cells infected with the recombinant virus secreted a functional LqhαIT polypeptide. Spodoptera littoralis and Heliothis armigera larvae injected with recombinant budded virus, showed typical intoxication symptoms. This recombinant virus showed enhanced insecticidal potency against H. armigera larvae compared with wild type AcNPV. The present expression system will facilitate: (1) the future elucidation of structural elements involved in its prominent anti‐insect toxicity; and (2) the future design of genetically modified alpha toxins with improved anti‐insect selectivity.Keywords
This publication has 10 references indexed in Scilit:
- Binding of an α scorpion toxin to insect sodium channels is not dependent on membrane potentialFEBS Letters, 1993
- Baculovirus Expression of Alkaline Phosphatase as a Reporter Gene for Evaluation of Production, Glycosylation and SecretionNature Biotechnology, 1992
- Nucleotide sequence and structure analysis of a cDNA encoding an alpha insect toxin from the scorpion Leiurus quinquestriatus hebraeusToxicon, 1991
- Insecticidal effects of an insect-specific neurotoxin expressed by a recombinant baculovirusVirology, 1991
- Venom neurotoxins — models for insectisidesPhytoparasitica, 1991
- Development of a Recombinant Baculovirus Expressing an Insect-Selective Neurotoxin: Potential for Pest ControlNature Biotechnology, 1991
- Construction of an improved baculovirus insecticide containing an insect-specific toxin geneNature, 1991
- A scorpion venom neurotoxin paralytic to insects that affects sodium current inactivation: purification, primary structure, and mode of actionBiochemistry, 1990
- Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDaAnalytical Biochemistry, 1987
- POLO: A New Computer Program for Probit AnalysisBulletin of the Entomological Society of America, 1977