Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc.
- 1 December 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (23) , 8885-8889
- https://doi.org/10.1073/pnas.85.23.8885
Abstract
Two-dimensional gel fractionation has revealed the existence of a number (.gtoreq.8) of additional species of HeLa cell small RNAs that have 5'' trimethylguanosine cap structures and are bound by proteins containing Sm epitopes. Therefore, these low-abundance (103-104 per cell) RNAs belong to the Sm class of small nuclear ribonucleoproteins (snRNPs), whose best-known members are the four highly abundant (.apprxeq. 106 per cell) particles required for pre-mRNA splicing. The complexity of Sm snRNPs in mammalian cells is thus not greatly different from that previously established for lower eukaryotes. Two of the new RNAs, designated U11 (131 nucleotides) and U12 (150 nucleotides), have been sequenced. The U11 and U12 snRNPs have been characterized further by examining their nuclease sensitivity and their possible interactions with other snRNPs. Potential roles for the low-abundance snRNPs in aspects of pre-mRNA processing are discussed.This publication has 37 references indexed in Scilit:
- Yeast contains small nuclear RNAs encoded by single copy genesCell, 1983
- Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.Proceedings of the National Academy of Sciences, 1981
- Two Novel Classes of Small Ribonucleoproteins Detected by Antibodies Associated with Lupus ErythematosusScience, 1981
- Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysisNucleic Acids Research, 1980
- Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosusProceedings of the National Academy of Sciences, 1979
- [3] Use of in Vitro32P labeling in the sequence analysis of nonradioactive tRNAsPublished by Elsevier ,1979
- 3′-Terminal labelling of RNA with T4 RNA ligaseNature, 1978
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Mapping adenines, guanines, and pyrimidines in RNANucleic Acids Research, 1977
- Purification and Characterization of Adenosine Triphosphate: Ribonucleic Acid Adenyltransferase from Escherichia coliEuropean Journal of Biochemistry, 1973