Patulin-induced ion flux in cultured renal cells and reversal by dithiothreitol and glutathione: A scanning electron microscopy (sem) X-ray microanalysis study

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Abstract
Patulin (PAT), a compound produced by certain species of Aspergillus, Penicillium, and Byssochlamys, is frequently found associated with agricultural commodities. PAT has many effects on membrane function, including the inhibition of the isolated Na+‐K+ ATPase. In this study, a scanning electron microscope equipped with an energy dispersive spectroscopy X‐ray microanalysis system was used to examine individual cultured renal epithelial cells (LLC‐PK1) in order to determine the effects of PAT on the relative intracellular ion concentrations. The estimated EC50 (60 min) for both sodium influx and potassium efflux was between 10 and 50 μm for ouabain. For PAT, the EC50 (60 min) was 250 μm for sodium influx and 100 μm for potassium efflux. However, 1 mM patulin at 240 min caused complete reversal of the sodium and potassium content of cells, and 1 mM ouabain at 240 min did not. The effect of patulin on sodium and potassium flux was both concentration and time dependent and was reversed by dithiothreitol and glutathione. PAT (250 μM) but not ouabain (250 μM) induced massive blebbing of LLC‐PK1 cells. Thus, the interaction of PAT with cellular membranes involves both alterations in the regulation of intracellular ion content and the cyto‐skeleton. We hypothesize that patulin alters intracellular ion content via Na+–K+ ATPase and non‐Na+–K+ ATPase mechanisms.