A three–dimensional model of the Rev–binding element of HIV–1 derived from analyses of aptamers
- 1 May 1994
- journal article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 1 (5) , 293-300
- https://doi.org/10.1038/nsb0594-293
Abstract
Coordinated variations in the sequence of the Rev-binding element of HIV-1, identified by in vitro genetic selections, have been used as distance and conformational constraints for molecular modelling. Three-dimensional models of the wild-type Rev-binding element and several, evolved RNA ligands (aptamers) have been constructed. These models demonstrate that non-Watson-Crick pairings open the major groove allowing access of an alpha-helical peptide from Rev, and explain why some selected RNA sequences can bind Rev more tightly than others.Keywords
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