The preparation of nucleated erythrocyte ghosts from avian erythrocytes

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Abstract
A method is described for the preparation of erythrocyte ghosts, which are haemoglobin‐depleted, from domestic fowl and turkey erythrocytes. These erythrocyte ghosts retain their ellipsoid shape and their nuclei, which are shown to possess the typical double nuclear membrane. In the cytoplasmic compartment of the ghosts, mitochondria have been clearly observed. There is little or no indication of endoplasmic reticulum. Filamentous structures have been seen adhering to the outer nuclear membrane, the mitochondria and the inner surface of the plasma membrane. These filaments may extend between the plasma membrane and the surfaces of the nucleus and mitochondria, thereby providing a form of structural anchorage for these organelles. It is a consistent observation that the nucleus of the intact erythrocyte and its ghost always occupies a central position, so some structure must be holding the nucleus otherwise one would expect to occasionally find it positioned off‐centre. Significantly, very few of the filament structures are observed at the extremities of the avian erythrocyte ghost, well away from the nucleus.

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