THE INDUCTION OF CELLULAR DNA SYNTHESIS BY SIMIAN VIRUS 40 IN CONTACT-INHIBITED AND IN X-IRRADIATED CELLS

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Abstract
It has been shown that SV40 can induce the synthesis of cellular DNA in contact-inhibited and in X-irradiated 3T3 cells. This induction was detected at a high multiplicity of infection (100 or 500 PFU per cell), but not at a lower multiplicity (40 PFU per cell). In contact-inhibited or X-irradiated BS-C-1 cells, where nearly all the cells synthesize virus, no induction of cellular DNA synthesis was detected after SV40 infection at either the higher or the lower multiplicity. The results indicate that SV40 can induce the synthesis of cellular DNA in 3T3 cells in the absence of detectable virus DNA synthesis; and that in contrast to mouse cells infected with polyoma virus where the major fraction of the induced DNA synthesis can be attributed to cellular DNA, there was no detectable induction of cellular DNA synthesis by SV40 in the virus-yielding BS-C-1 cells.