MAPPING DNA-SEQUENCES IN A HUMAN X-CHROMOSOME DELETION WHICH EXTENDS ACROSS THE REGION OF THE DUCHENNE MUSCULAR-DYSTROPHY MUTATION

  • 1 January 1985
    • journal article
    • research article
    • Vol. 37  (3) , 451-462
Abstract
A somatic cell hybrid was constructed and characterized using fibroblasts from a phenotypically normal women who possesses an X chromosome with an interstitial deletion of the short arm. High-resolution banding indicates that the deleted segment is either Xp22.13-p11.4 or Xp22.11-p11.23. Southern blot hybridization to previously mapped DNA sequences confirms that the missing segment of the X chromosome is a deletion and not an interstitial translocation and supports the cytogenetic interpretation that the deletion extends proximal of Xp11.3 and therefore probably comprises Xp22.11-p11.23. Three further DNA sequences were localized to the region of the deleted segment. The following order was assigned to the 7 probes used: Xpter-RC8-pXUT22-(OA1,C7,M2C)-L1.28-RD6-Xcen.