2‐hydroxyamino‐1‐methyl‐6‐phenylimidazo [4,5‐b] pyridine induction of recombinational mutations in mammalian cell lines as detected by dna fingerprinting

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Abstract
2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (PhIP) is the most abundant mutagenic heterocyclic amine in cooked foods. Two mouse tumor cell lines, BMT11 and FM3A, were exposed to its proximate form, 2‐hydroxyamino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (N‐OH‐PhIP). Fifty‐six subclones of BMT11 and 39 subclones of FM3A were isolated and analyzed by DNA fingerprinting. Treatment with 10–20 μM N‐OH‐PhIP gave rise to extra bands or shifted bands, but treatment without N‐OH‐PhIP did not. This suggests that mutations resulting from recombination were induced. The mutation frequencies were 21–53% and 22–35% for BMT11 and FM3A, respectively. These findings suggest that PhIP induces recombinational mutations.

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