SELECTIVE CHEMOTAXIS OF IA ANTIGEN-POSITIVE MACROPHAGES BY MACROPHAGE CHEMOTACTIC LYMPHOKINE PRODUCED BY CONCANAVALIN-A STIMULATION

  • 1 January 1984
    • journal article
    • research article
    • Vol. 52  (4) , 641-647
Abstract
M1- cells (leukemic cell line cells established from SL/Am mouse), Mm1 cells (macrophage-like cells subcloned from M1- cells) and undifferentiated B24 cells (subcloned from M1- cells) were similarly Ia-negative and showed little chemotactic response to macrophage chemotactic lymphokine (MCF-LD) produced by Con A [concanavalin A] stimulation of spleen cells. When M1- cells and B24 cells were differentiated by differentiating factor in the fibroblast-conditioned medium, they became positive for surface Ia antigens and exhibited chemotactic response to MCF-LD. Treatment of the cell-line cells and mouse peritoneal exudate macrophages with anti-Ia antiserum and complement reduced the number of cells responsive to MCF-LD. When comparing the chemotactic responses to MCF-LD of Ia-negative and Ia-positive peritoneal exudate macrophages, separated by electric programmable individual cell sorter, Ia-positive cells responded more strongly than Ia-negative cells.