A macrophage chemotactic factor sharing common antigenicity with immunoglobulin G from DNP-ascaris extract-induced skin lesion in guinea-pig

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Abstract
A macrophage chemotactic factor (factor a) was extracted from DNP[dinitrophenyl]-Ascaris extract-induced skin lesions (showing a maximal macrophage reaction) in guinea pigs and then purified by gel filtration on Sephadex G-100 and chromatography using DEAE-Sephadex and CM-Sephadex. This material was a thermolabile protein (free of nucleic acid) with a MW of about 150,000. It shared common antigenicity with serum Ig[immunoglobulin]G, and its chemotactic activity was completely absorbed by anti-IgG and anti-L chain antibodies, but not by anti-BSA [bovine serum albumin] antibody. In contrast to leucoegresin, this material seemed active for macrophages but not for neutrophils. Less active factors b and c were thermostable and not absorbed by the antibodies described above. The ratio of chemotactic activity of factors a, b and c in the skin extracts was roughly 60:25:15.