Identification of Specific Antibodies Prepared Against Purified Immobilization Antigens of Paramecium aurelia

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Abstract
SYNOPSIS The identity of antibodies prepared against preparations of isolated immobilization antigen protein of Paramecium aurelia has been studied. Antisera were fractionated by ammonium sulfate precipitation, sucrose gradient centrifugation, and ion exchange chromatography on DEAE cellulose. The different fractions were assayed for the presence of specific antibodies by the immobilization test, and the globulins were identified by immuno‐electrophoresis. It is concluded that in the sera studied in this investigation, specific antibodies are excusively of the class Ig G.