Raf-1 is a potential substrate for mitogen-activated protein kinase in vivo
- 15 July 1991
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 277 (2) , 573-576
- https://doi.org/10.1042/bj2770573
Abstract
MAP (mitogen-activated protein) kinase is shown to phosphorylate baculovirally expressed Raf-1 in vitro, generating one major tryptic phosphopeptide which co-migrated with a peptide from Raf-1 32P-labelled in situ. This peptide also undergoes an insulin-dependent increase in labelling. Thus the serine/threonine kinase Raf-1 may be a substrate for MAP kinase in vivo.Keywords
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