Detection of immunoperoxidase labelled mycoplasmas in cell culture by light microscopy and electronmicroscopy

Publisher Website
Google Scholar
Abstract
A McCoy [mouse] cell line persistently infected with M. orale was examined by light microscopy and EM after specific labeling with a direct immunoperoxidase conjugate. Mycoplasmas were readily detected in monolayer cultures in bright-field conditions; these were related to labeled organisms observed by EM in thin sections of similar cells. The specificity of the conjugate for M. orale was demonstrated by blocking with unconjugated antiserum and by its inability to detect M. bovirhinis. Non-specific background labeling was consistently absent.