CONTINUOUS-FLOW ANALYSIS FOR GLUCOSE IN SERUM, WITH USE OF HEXOKINASE AND GLUCOSE-6-PHOSPHATE-DEHYDROGENASE CO-IMMOBILIZED IN TUBULAR FORM

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (1) , 123-129
Abstract
Hexokinase and glucose-6-phosphate dehydrogenase, co-immobilized at the inner wall of plastic tubing, were used with continuous-flow analyzers to measure [human] serum glucose. The performance of the enzyme-containing coils meets the hydraulic requirements of the continuous-flow instruments, and the average carryover does not exceed 2.2%. A glucose concentration change of 1 g/l yields a 0.08 change in absorbance. There is no significant deviation from linearity for glucose concentrations up to 5 g/l. The method is similar to the Reference Method proposed by the Food and Drug Administration, and results by the 2 methods agree and correlate well (r = 0.999). The operational stability of the enzyme tubes is sufficient to allow analysis of at least 10,000 serum samples. The effect of reagent concentrations and temperature on the performance of the enzyme coils was investigated; the results shed light on the nature of the heterogeneous dual-enzymic reaction.