SPORULATION REQUIREMENTS OF BACILLUS COAGULANS VAR. THERMOACIDURANS IN COMPLEX MEDIA

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Abstract
On agar media containing peptone, growth as well as sporulation is markedly stimulated by the addition of 1 ppm or more of manganous sulfate, nickel sulfate or cobalt sulfate, particularly when the cultures are incubated at 55[degree]C. Sporulation ability degenerates following serial transfer in thermoacidurans broth. Subsequent culturing on peptone media containing added Mn stimulates sporulation but fails to restore the culture to its original ability to sporulate. In thermoacidurans broth shake culture, the addition of Mn alone stimulated a high degree of sporulation by strains 8038 and 1B but not for strain 43P-1. With the latter strain, it was necessary to add, in addition to Mn, intermediates related to the citric acid cycle. Malic and alpha -ketoglutaric acid were the most effective but succinic and isocitric acid and alanine also provided a definite stimulation. It is presumed that the added acids "triggered" the citric acid cycle to provide additional energy required for the sporulation process.
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