A suitable method for construction and cloning hybrid plasmids containingEcoRI-fragments ofE. coli genome
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 150 (2) , 211-219
- https://doi.org/10.1007/bf00695401
Abstract
A convenient procedure for the isolation of specificEcoRI-fragments ofE. coli genome and their amplification on Km-resistance plasmid vector CK Δ11 is described. The hybrid molecules were constructedin vitro usingEcoRI-digestion, followed by ligation. Then appropriatedE. coli strain was transformed with ligated DNA mixture and hybrid plasmids CK Δ11-arg +, CK Δ11-his +, CK Δ11-thr + and CK δ11-leu + containing loci ofE. coli genome were selected by molecular cloning. The hybrid plasmids obtained consisted of oneEcoRI-fragment of initial plasmid CK Δ11 and one respective specific portion ofE. coli genome.This publication has 17 references indexed in Scilit:
- A rellable technique for large-scale DNA separationAnalytical Biochemistry, 1977
- Studies of mouse mitochondrial DNA in escherichia coli: Structure and function of the eucaryotic-procaryotic chimeric plasmidsCell, 1975
- Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structuresJournal of Molecular Biology, 1975
- Characterization of six cloned DNAs from drosophila melanogaster, including one that contains the genes for rRNACell, 1975
- Studies on the cleavage of bacteriophage lambda DNA with EcoRI restriction endonucleaseJournal of Molecular Biology, 1975
- Polynucleotides. CXXIII. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymeraseBiochemistry, 1973
- Formation of complex Col E1 DNA by replicationBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970
- SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORMProceedings of the National Academy of Sciences, 1969
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961