Partial sequence of the purified protein confirms the identity of cDNA coding for human lysosomal α-mannosidase B
- 15 January 1995
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 305 (2) , 363-366
- https://doi.org/10.1042/bj3050363
Abstract
Human lysosomal alpha-mannosidase has been purified by a simple and rapid method in sufficient quantities for the analysis of its subunit composition and partial protein sequencing. Analysis of the N-terminal residues of the 30 kDa polypeptide has enabled us to confirm the identity of the recently cloned cDNA that was tentatively identified as that of lysosomal alpha-mannosidase [Nebes and Schmidt (1994) Biochem. Biophys. Res. Commun. 200, 239-245] and to locate the position of this polypeptide within the total deduced amino acid sequence. This finding will therefore provide a firm foundation for the characterization of alpha-mannosidosis mutations.Keywords
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