A Process for large‐scale isolation of β‐galactosidase from E. coli in an aqueous two‐phase system
- 1 July 1983
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 25 (7) , 1789-1800
- https://doi.org/10.1002/bit.260250709
Abstract
A method suitable for large-scale isolation of β-galactosidase from a suspension of disintegrated E. coli cells has been developed. In an aqueous two-phase system consiting of PEG 6000 and potassium phosphate, all cell debris and the major part of the proteins and nucleic acids were partitioned to the denser salt phase. Seventy-five percent of the β-galactosidase was recovered in the lighter PEG phase, giving a purification ratio of about 12.Keywords
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