β-Adrenergic Agonist Induced Androgen Production during Primary Culture of Mouse Leydig Cells

Publisher Website
Google Scholar
Abstract
Mouse testicular interstitial cells and Leydig cells prepared by density-gradient centrifugation developed the ability to increase androgen production in response to isoproterenol between 24 and 48 h of primary culture. Although exposure of the cultures to isoproterenol or cycloheximide inhibited the acquisition of responsiveness to .beta.-adrenergic stimulation, in vivo treatment with propranolol did not alter the time at which cultures became responsive to isoproterenol. The acquisition of .beta.-agonist responsiveness during culture is not a recovery from .beta.-adrenergic desensitization.