Specific termination of RNA polymerase synthesis as a method of RNA and DNA sequencing
Open Access
- 1 October 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (10) , 3549-3564
- https://doi.org/10.1093/nar/5.10.3549
Abstract
Termination of RNA synthesis with 3′-O-Methylnucleoside 5′-triphosphates have been studied using E.coli RNA polymerase holoenzyme and poly [d(A-T)] as well as unfractionated T7 D Δ III DNA as templates. It was shown that the termination can be used for DNA sequencing. A sequence of a part of RNA synthesized from AI promoter of the DNA have been determined. Syntheses of four 3′-O-Methylnucleoside 5′-triphosphates are described.Keywords
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